The Hedgehog signaling pathway is important during embryonic development, and in the adult organism in maintaining tissue homeostasis. The discovery of aberrant activation of the Hedgehog pathway in melanoma, one of the most aggressive tumors, meant potentially directing new therapies and melanoma diagnostics to the components of this pathway and to the genes and proteins that this signaling pathway regulates. In this graduate thesis, the expression of GLI1 and S100A7 proteins was analyzed on melanoma samples from patients and on melanoma and skin keratinocytes cell lines. The main objective was to examine whether there is a connection in the expression of these two proteins in melanoma tissues, in order to determine whether GLI1 regulates the expression of S100A7. The expression of the transcription factor GLI1, a component of the Hedgehog pathway, and its target, the protein S100A7, were detected mainly in the epithelium surrounding the tumor, while their expression in the tumors themselves was weak. The staining intensity of both proteins increases with the stage of the disease. Immunofluorescence on cell lines further confirmed the increase in S100A7 protein expression in dependence on GLI1 protein expression in melanoma and skin keratinocyte cell models. GLI1 and S100A7 proteins are an important component of melanoma development, so new research focused on these proteins could lead to their use as potential prognostic markers, as well as to the development of new drugs.