prikaz prve stranice dokumenta The role of ATF3 in neuronal differentiation and development of neuronal networks in opossum postnatal cortical cultures
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The role of ATF3 in neuronal differentiation and development of neuronal networks in opossum postnatal cortical cultures
International Journal of Molecular Sciences, 23 (2022), 9; 4964. https://doi.org/10.3390/ijms23094964
Petrović, Antonela; Ban, Jelena; Ivanićić, Matea; Tomljanović, Ivana; Mladinic, Miranda

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Petrović, A., Ban, J., Ivanićić, M., Tomljanović, I. i Mladinic, M. (2022). The role of ATF3 in neuronal differentiation and development of neuronal networks in opossum postnatal cortical cultures. International Journal of Molecular Sciences, 23. (9). doi: 10.3390/ijms23094964

Petrović, Antonela, et al. "The role of ATF3 in neuronal differentiation and development of neuronal networks in opossum postnatal cortical cultures." International Journal of Molecular Sciences, vol. 23, br. 9, 2022. https://doi.org/10.3390/ijms23094964

Petrović, Antonela, Jelena Ban, Matea Ivanićić, Ivana Tomljanović i Miranda Mladinic. "The role of ATF3 in neuronal differentiation and development of neuronal networks in opossum postnatal cortical cultures." International Journal of Molecular Sciences 23, br. 9 (2022). https://doi.org/10.3390/ijms23094964

Petrović, A., et al. (2022) 'The role of ATF3 in neuronal differentiation and development of neuronal networks in opossum postnatal cortical cultures', International Journal of Molecular Sciences, 23(9). doi: 10.3390/ijms23094964

Petrović A, Ban J, Ivanićić M, Tomljanović I, Mladinic M. The role of ATF3 in neuronal differentiation and development of neuronal networks in opossum postnatal cortical cultures. International Journal of Molecular Sciences [Internet]. 29.04.2022. [pristupljeno 14.04.2025.];23(9). doi: 10.3390/ijms23094964

A. Petrović, J. Ban, M. Ivanićić, I. Tomljanović i M. Mladinic, "The role of ATF3 in neuronal differentiation and development of neuronal networks in opossum postnatal cortical cultures", International Journal of Molecular Sciences, vol. 23, br. 9, Travanj 2022. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:193:724422. [Citirano: 14.04.2025.]

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Naslov (engleski)The role of ATF3 in neuronal differentiation and development of neuronal networks in opossum postnatal cortical cultures
AutorAntonela Petrović
AutorJelena Ban
AutorMatea Ivanićić
AutorIvana Tomljanović
AutorMiranda Mladinic
Autorova ustanovaSveučilište u Rijeci
(Fakultet biotehnologije i razvoja lijekova)
Znanstveno / umjetničko
područje, polje i grana		INTERDISCIPLINARNA PODRUČJA ZNANOSTI
Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)
Sažetak (engleski)
Activating transcription factor 3 (ATF3), a member of the ATF/cAMP response element binding (CREB) family, is upregulated by various intracellular and extracellular signals such as injury and signals related to cell proliferation. ATF3 also belongs to the regeneration-associated genes (RAG) group of transcription factors. RAG and ATF/CREB transcription factors that play an important role in embryonic neuronal development and PNS regeneration may also be involved in postnatal neuronal... Više differentiation and development, as well as in the regeneration of the injured CNS. Here we investigated the effect of ATF3 in differentiation, neural outgrowth, network formation, and regeneration after injury using postnatal dissociated cortical neurons derived from neonatal opossums (Monodelphis domestica). Our results show that RAG and ATF genes are differentially expressed in early differentiated neurons versus undifferentiated neurospheres and that many members of those families, ATF3 in particular, are upregulated in cortical cultures obtained from younger animals that have the ability to fully functionally regenerate spinal cord after injury. In addition, we observed different intracellular localization of ATF3 that shifts from nuclear (in neuronal progenitors) to cytoplasmic (in more mature neurons) during neuronal differentiation. The ATF3 inhibition, pharmacological or by specific antibody, reduced the neurite outgrowth and differentiation and caused increased cell death in early differentiating cortical neuronal cultures, suggesting the importance of ATF3 in the CNS development of neonatal opossums. Finally, we investigated the regeneration capacity of primary cortical cultures after mechanical injury using the scratch assay. Remarkably, neonatal opossum-derived cultures retain their capacity to regenerate for up to 1 month in vitro. Inhibition of ATF3 correlates with reduced neurite outgrowth and regeneration after injury. These results indicate that ATF3, and possibly other members of RAG and ATF/CREB family of transcription factors, have an important role both during cortical postnatal development and in response after injury. Sakrij dio sažetka
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran - međunarodna recenzija
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaInternational Journal of Molecular Sciences
Brojčani podacivol. 23, br. 9, 4964
p-ISSN1422-0067
DOIhttps://doi.org/10.3390/ijms23094964
URN:NBNurn:nbn:hr:193:724422
Datum objave publikacije2022-04-29
ProjektŠifra: IP-2016-06-7060
Naziv (hrvatski): Istraživanje granice između neurodegeneracije i neuroregeneracije: identifikacija ključnih molekula pomoću proteomike i funkcionalnih testova na leđnoj moždini sisavaca
Kratica: DefineREgenAgeMode
Voditelj: Miranda Mladinić Pejatović
Pravna nadležnost: Hrvatska
Financijer: HRZZ
Linija financiranja: IP
URL dokumentahttps://www.mdpi.com/1422-0067/23/9/4964
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje 3.0 (CC BY 3.0)
Datum i vrijeme pohrane2022-07-13 11:28:37
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