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Virus Progeny of Murine Cytomegalovirus Bacterial Artificial Chromosome pSM3fr Show Reduced Growth in Salivary Glands due to a Fixed Mutation of MCK-2
Journal of Virology, 85 (2011), 19; 10346-10353. https://doi.org/10.1128/JVI.00545-11
Jordan, Stefan; Krause, Johannes; Prager, Adrian; Mitrović, Maja; Jonjić, Stipan; Koszinowski, Ulrich H.; Adler, Barbara

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Jordan, S., Krause, J., Prager, A., Mitrović, M., Jonjić, S., Koszinowski, U. H. i Adler, B. (2011). Virus Progeny of Murine Cytomegalovirus Bacterial Artificial Chromosome pSM3fr Show Reduced Growth in Salivary Glands due to a Fixed Mutation of MCK-2. Journal of Virology, 85. (19), 10346-10353. doi: 10.1128/JVI.00545-11

Jordan, Stefan, et al. "Virus Progeny of Murine Cytomegalovirus Bacterial Artificial Chromosome pSM3fr Show Reduced Growth in Salivary Glands due to a Fixed Mutation of MCK-2." Journal of Virology, vol. 85, br. 19, 2011, str. 10346-10353. https://doi.org/10.1128/JVI.00545-11

Jordan, Stefan, Johannes Krause, Adrian Prager, Maja Mitrović, Stipan Jonjić, Ulrich H. Koszinowski i Barbara Adler. "Virus Progeny of Murine Cytomegalovirus Bacterial Artificial Chromosome pSM3fr Show Reduced Growth in Salivary Glands due to a Fixed Mutation of MCK-2." Journal of Virology 85, br. 19 (2011): 10346-10353. https://doi.org/10.1128/JVI.00545-11

Jordan, S., et al. (2011) 'Virus Progeny of Murine Cytomegalovirus Bacterial Artificial Chromosome pSM3fr Show Reduced Growth in Salivary Glands due to a Fixed Mutation of MCK-2', Journal of Virology, 85(19), str. 10346-10353. doi: 10.1128/JVI.00545-11

Jordan S, Krause J, Prager A, Mitrović M, Jonjić S, Koszinowski UH, i sur.. Virus Progeny of Murine Cytomegalovirus Bacterial Artificial Chromosome pSM3fr Show Reduced Growth in Salivary Glands due to a Fixed Mutation of MCK-2. Journal of Virology [Internet]. 03.08.2011. [pristupljeno 16.04.2024.];85(19):10346-10353. doi: 10.1128/JVI.00545-11

S. Jordan, et al., "Virus Progeny of Murine Cytomegalovirus Bacterial Artificial Chromosome pSM3fr Show Reduced Growth in Salivary Glands due to a Fixed Mutation of MCK-2", Journal of Virology, vol. 85, br. 19, str. 10346-10353, Kolovoz 2011. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:184:976493. [Citirano: 16.04.2024.]

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Naslov (engleski)Virus Progeny of Murine Cytomegalovirus Bacterial Artificial Chromosome pSM3fr Show Reduced Growth in Salivary Glands due to a Fixed Mutation of MCK-2
AutorStefan Jordan
AutorJohannes Krause
AutorAdrian Prager
AutorMaja Mitrović
AutorStipan Jonjić
AutorUlrich H. Koszinowski
AutorBarbara Adler
Autorova ustanovaSveučilište u Rijeci
Medicinski fakultet
(Katedra za histologiju i embriologiju)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Temeljne medicinske znanosti
Sažetak (engleski)
Murine cytomegalovirus (MCMV) Smith strain has been cloned as a bacterial artificial chromosome (BAC) named pSM3fr and used for analysis of virus gene functions in vitro and in vivo. When sequencing the complete BAC genome, we identified a frameshift mutation within the open reading frame (ORF) encoding MCMV chemokine homologue MCK-2. This mutation would result in a truncated MCK-2 protein. When mice were infected with pSM3fr-derived virus, we observed reduced virus production in salivary glands, which could be reverted by repair of the frameshift mutation. When looking for the source of the mutation, we consistently found that virus stocks of cell culture-passaged MCMV Smith strain are mixtures of viruses with or without the MCK-2 mutation. We conclude that the MCK-2 mutation in the pSM3fr BAC is the result of clonal selection during the BAC cloning procedure.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran - međunarodna recenzija
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaJournal of Virology
Brojčani podacivol. 85, br. 19, str. 10346-10353
p-ISSN0022-538X
DOIhttps://doi.org/10.1128/JVI.00545-11
URN:NBNurn:nbn:hr:184:976493
Datum objave publikacije2011-08-03
Datum objave publikacije2011-10-01
ProjektŠifra: 062-0621261-1263
Naziv (hrvatski): Molekularni mehanizmi citomegalovirusnog izmicanja imunološkom nadzoru
Voditelj: Stipan Jonjić
Pravna nadležnost: Hrvatska
Financijer: MZOS
Linija financiranja: ZP
URL dokumentahttp://jvi.asm.org/cgi/doi/10.1128/JVI.00545-11
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje-Nekomercijalno-Bez prerada 4.0 međunarodna (CC BY-NC-ND 4.0)
Datum i vrijeme pohrane2018-11-09 08:27:51