Oxygen therapy is the use of oxygen at concentrations greater than that in the air (21%) for the treatment or prevention of hypoxia. The purpose is to increase oxygen saturation in tissues where saturation levels are too low due to illness or injury. Nursing tasks in the use of oxygen therapy are the recognition of indications for the use of oxygen therapy, oxygen supply, preparation of oxygen supplies, the application of oxygen therapy, patient observation and SpO₂ monitoring. The objective of the research work are to test the acquired knowledge about the application of oxygen therapy and how much experience the students have gained with the same during the study. The student questionnaire was an anonymous online survey questionnaire titled "The Application of Oxygen Therapy Knowledge and Experience Survey". The students were second and third year students at a nursing study at the Faculty of Health Sciences University of Rijeka. The results of the research have shown that students at the Faculty of Health Sciences have sufficient knowledge of oxygen therapy and that most of the students applied oxygen therapy during their studies. The study was conducted for the frequent application of oxygen therapy at the nurses / technicians workshops where most students will be after graduation.