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Comprehensive analysis of varicella-zoster virus proteins using a new monoclonal antibody collection.
Journal of virology, 87 (2013), 12; 6943-6954. https://doi.org/10.1128/JVI.00407-13
Roviš, Tihana Lenac; Bailer, Susanne M.; Pothineni, Venkata R.; Ouwendijk, Werner J. D.; Šimic, Hrvoje; Babić, Marina; Miklić, Karmela; Malić, Suzana; Verweij, Marieke C.; Baiker, Armin; Gonzalez, Orland; von Brunn, Albrecht; Zimmer, Ralf; Frueh, Klaus; Verjans, Georges M. G. M.; Jonjić, Stipan; Haas, Jürgen Više autora...

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Roviš, T. L., Bailer, S. M., Pothineni, V. R., Ouwendijk, W. J. D., Šimic, H., Babić, M. ... Haas, J. (2013). Comprehensive analysis of varicella-zoster virus proteins using a new monoclonal antibody collection.. Journal of virology, 87. (12), 6943-6954. doi: 10.1128/JVI.00407-13

Roviš, Tihana Lenac, et al. "Comprehensive analysis of varicella-zoster virus proteins using a new monoclonal antibody collection.." Journal of virology, vol. 87, br. 12, 2013, str. 6943-6954. https://doi.org/10.1128/JVI.00407-13

Roviš, Tihana Lenac, Susanne M. Bailer, Venkata R. Pothineni, Werner J. D. Ouwendijk, Hrvoje Šimic, Marina Babić, Karmela Miklić, et al. "Comprehensive analysis of varicella-zoster virus proteins using a new monoclonal antibody collection.." Journal of virology 87, br. 12 (2013): 6943-6954. https://doi.org/10.1128/JVI.00407-13

Roviš, T. L., et al. (2013) 'Comprehensive analysis of varicella-zoster virus proteins using a new monoclonal antibody collection.', Journal of virology, 87(12), str. 6943-6954. doi: 10.1128/JVI.00407-13

Roviš TL, Bailer SM, Pothineni VR, Ouwendijk WJD, Šimic H, Babić M, i sur.. Comprehensive analysis of varicella-zoster virus proteins using a new monoclonal antibody collection.. Journal of virology [Internet]. 2013. [pristupljeno 14.04.2024.];87(12):6943-6954. doi: 10.1128/JVI.00407-13

T. L. Roviš, et al., "Comprehensive analysis of varicella-zoster virus proteins using a new monoclonal antibody collection.", Journal of virology, vol. 87, br. 12, str. 6943-6954, 2013. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:184:359337. [Citirano: 14.04.2024.]

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Naslov (engleski)Comprehensive analysis of varicella-zoster virus proteins using a new monoclonal antibody collection.
AutorTihana Lenac Roviš
AutorSusanne M. Bailer
AutorVenkata R. Pothineni
AutorWerner J. D. Ouwendijk
AutorHrvoje Šimic
AutorMarina Babić
AutorKarmela Miklić
AutorSuzana Malić
AutorMarieke C. Verweij
AutorArmin Baiker
AutorOrland Gonzalez
AutorAlbrecht von Brunn
AutorRalf Zimmer
AutorKlaus Frueh
AutorGeorges M. G. M. Verjans
AutorStipan Jonjić
AutorJürgen Haas
Autorova ustanovaSveučilište u Rijeci
Medicinski fakultet
(Centar za proteomiku)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Temeljne medicinske znanosti
Sažetak (engleski)
Varicella-zoster virus (VZV) is the etiological agent of chickenpox and shingles. Due to the virus's restricted host and cell type tropism and the lack of tools for VZV proteomics, it is one of the least-characterized human herpesviruses. We generated 251 monoclonal antibodies (MAbs) against 59 of the 71 (83%) currently known unique VZV proteins to characterize VZV protein expression in vitro and in situ. Using this new set of MAbs, 44 viral proteins were detected by Western blotting (WB) and indirect immunofluorescence (IF); 13 were detected by WB only, and 2 were detected by IF only. A large proportion of viral proteins was analyzed for the first time in the context of virus infection. Our study revealed the subcellular localization of 46 proteins, 14 of which were analyzed in detail by confocal microscopy. Seven viral proteins were analyzed in time course experiments and showed a cascade-like temporal gene expression pattern similar to those of other herpesviruses. Furthermore, selected MAbs tested positive on human skin lesions by using immunohistochemistry, demonstrating the wide applicability of the MAb collection. Finally, a significant portion of the VZV-specific antibodies reacted with orthologs of simian varicella virus (SVV), thus enabling the systematic analysis of varicella in a nonhuman primate model system. In summary, this study provides insight into the potential function of numerous VZV proteins and novel tools to systematically study VZV and SVV pathogenesis.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran - međunarodna recenzija
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaJournal of virology
Brojčani podacivol. 87, br. 12, str. 6943-6954
e-ISSN1098-5514
DOIhttps://doi.org/10.1128/JVI.00407-13
URN:NBNurn:nbn:hr:184:359337
Datum objave publikacije2013
ProjektŠifra: 229585
Naziv (engleski): The Center for Antibody Production Rijeka: Upgrading the Central Research and Service Infrastructure for the South Eastern Region of Europe
Kratica: CAPRI2010
Pravna nadležnost: eu
Financijer: EC
Linija financiranja: FP7
URL dokumentahttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3676123/
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Zaštićeno autorskim pravom.
Datum i vrijeme pohrane2017-06-21 07:18:58