Introduction: Nursing has gone through a long developmental path from a primitive profession to a modern and noble profession, and thus the perception of the nursing profession has changed. Aim: To examine the perception of the profession of a nurse among students in Split. Methods: The research was conducted at 5 faculties in Split: the University Department of Health Studies, the Faculty of Medicine, the Faculty of Law, the Faculty of Maritime Studies, the Faculty of Catholic Theology, and included 516 students. The questionnaire was completed anonymously, online using Google Forms from November 2020 to March 2021. Results: Attitudes about nursing as a profession between 5 faculties differ significantly (P <0.001), and the most positive attitudes about nursing as a profession were students of the Faculty of Medicine (X̄= 0.28; SD = 0.61), followed by students of the University Department of Health study (X̄= 0.18; SD = 0.97), while the students of the Catholic Theological Faculty had the worst attitudes about nursing as an occupation (X̄= -0.98; SD = 1.42). In the answers on respect for nursing as a profession among 5 study fields of the University Department of Health Studies (P <0.001), students of midwifery showed the highest level of respect for nursing as a profession (X̄= 0.39; SD = 0.88), while students of nursing showed the worst the level of respect for nursing as a profession (X̄= -0.52; SD = 1.06). Conclusions: Students of the University Department of Health Studies, Faculty of Medicine, and Catholic Theological Faculty have a more positive perception of the profession of a nurse than students of the Faculty of Law and the Faculty of Maritime Studies. Students of nursing and midwifery do not have a more positive perception of the profession of nursing than students of physiotherapy, medical-laboratory diagnostics, and radiological technology. Students who have friends or relatives who practice nursing have a partially more positive perception than students who do not. The desire to help, as the reason why they enrolled in the faculty, is most pronounced among the students of the Catholic Theological Faculty.