Nepafenac is a novel non-steroidal anti-inflammatory analgesic, cyclooxygenase inhibitor. Its therapeutic indications are prevention and treatment of postoperative pain and inflammation associated with cataract surgery and reduction in the risk of postoperative macular oedema associated with cataract surgery in diabetic patients. In this thesis, the electrochemical properties of nepafenac in electrolytes of various pH and on various electrodes with nanomaterial-modified surfaces were investigated by the use of cyclic and square-wave voltammetry. The goal of this thesis was to look into the electrochemical behaviour of nepafenac on various electrodes and to ascertain if the gathered results might prove useful in the development of an analytical method for the identification and quantification of the drug. Cyclic voltammetry measurements have established that the pH of 6 of the supporting electrolyte, that is, BR buffer, is the most efficient for the nepafenac oxidation peak intensity, which is seen at the potential of 0,80 V. Moreover, it has been proven that the oxidation process is irreversible. Out of various electrodes, a 3 mm-diameter glassy carbon electrode has shown the highest sensitivity with the least impact of the background current. By modifying the surface of the glassy carbon electrode with suspensions of various nanomaterials in 0,3% ethanolic solution of Nafion, higher drug peak intensity is achieved, which leads to higher sensitivity of the method. By using carbon nanofibers and graphene nanoplatelets, the nepafenac oxidation peak increases threefold compared to the nonmodified electrode, whereas by using graphene oxide there is less increase compared to the other two nanomaterials. Square-wave voltammetry scans have shown adsorption of the drug and its oxidation product on the surface of the nanomaterial-modified electrodes. The peak of the oxidation product is observed on the potential of 0,15 V even after scanning the electrode in the buffer, which implies strong adsorption or a possible chemical reaction with the surface of the electrode. Adsorption of the drug occurs in all three nanomaterials, but the adsorption of the oxidation product is significantly lesser on graphene nanoplatelets. In regard to adsorption kinetics, the adsorption-desorption equilibrium is established more rapidly on the electrode modified with graphene nanoplatelets than on the one modified with carbon nanofibers.