Available literature clearly shows the connection between the carbohydrate metabolism, inflammatory bowel disease (IBD) and diabetes, as well as the relevance of glucose transporters in these conditions. The aim of this work is to determine the expression of the transporters included in the transport of glucose across the intestinal barrier: Sodium Glucose transporter 1 (SGLT1), Glucose transporter 2 (GLUT2), and Sodium/pottasium ATPase (Na/K ATPase) in the mouse Dextrane Sodium Sulfate (DSS) model using Enzyme-Linked Immunosorbent Assay (ELISA). The effect of Cyclosporyne A, a pharmacological standard, on the expression of the listed transporters will be measured as well. Male C57BL6 mice were allocated in three groups: control vehicle (0.5% methyl cellulose), vehicle treated group challenged with 3% DSS and finally the group challenged with 3% DSS and treated with Cyclosporine A (80 mg/kg/PO/QD). During the course of the study body weight loss, stoll consistency and colorectal bleeding were measured and the sampling was performed on day 8 of the study. Colon length, haemathologic and histopathologic parameters were also measured. The concentrations of insulin and serum amiloid A protein in the serum, as well as the expression of GLUT2, SGLT1 and Na/K ATP-ase were measured by ELISA. Mann – Whitney U test (p < 0,05) was used to calculate statistical significance. The group treated with 3% DSS showed significant increase of stool consistency, colorectal bleeding, disease activity index and Nishitani scores when compared to the control group, while the group treated with Cyclosporine A showed significant reduction of these scores. Haematological parameters were decreased, while total leukocyte count, as well as subpopulation counts were increased in the DSS challenged group. The DSS group showed lower concentrations of glucose while the insulin concentration was increased. No significant change of the SGLT1 expression was measured in the small intestines, while GLUT2 showed increased and decreased expression in duodenum and jejunum, respectively, when compared to control group. Significant increase in the expression of Na/K ATP-ase was observed in jejunum and colon in the group of animals treated with DSS. These results support current knowledge regarding the link between the carbohydrates and IBD, however more detailed analyses are required in order to clearly describe the role of glucose transporters in the DSS model of IBD.