Introduction. Muscle interplay is a mechanism determined by the recruitment order of agonists and synergists that results from their onset times. The aim of this study was to examine the acute and prolonged effects of three different techniques of Kinesio Taping method, compared to the control procedures, and furthermore to find which technique is most effective in optimizing the muscle interplay within the lumbo-pelvic-hip complex, with the potential for primary prevention of pathological manifestations in the mentioned area. Methods. The sample consisted of 70 healthy subjects of both genders, randomly divided into 5 equal groups. The study included 6 dependent variables assessed through electromyographic, goniometric and trigonometric evaluation based on 4 different tests. These variables were: onsets of the ipsilateral/contralateral erector spinae muscles in relation to semitendinosus muscle of the tested leg, recruitment order of these muscles (Prone Hip Extension test), hip extension/flexion range of motion (Modified Thomas/Active Straight Leg Raise test) and the pelvic tilt angle. All measurements were performed at three timepoints: pre-intervention, 60 min after the intervention, and 48 h post-intervention. Results. A statistically significant interaction between the group and the timepoint was found by a mixed ANOVA for the variable onset of the contralateral erector spinae and for the range of motion variables. No statistically significant difference was found between the timepoints for the control groups, while significant acute effects were found for the experimental ones. Prolonged effects have also been found but have not been shown to be significantly different than acute effects. Two Kinesio Taping muscle techniques showed a different tendency towards the change of muscle activity, with the facilitation technique being more effective. The Kinesio Taping functional correction technique showed the greatest acute muscle interplay improvement, which was maintained at an approximately equal level for a prolonged time. Conclusion. The Kinesio Taping method could optimize muscle interplay, in which the most effective Kinesio Taping technique is the functional correction. Given the insignificance of the differences in the effects of Kinesio Taping techniques, we suggest the functional correction technique to achieve postural correction, which could optimize the muscle interplay immediately after the intervention. The muscle facilitation technique can also be useful to achieve the same goal in a prolonged time, while being, by its myofascial repercussions, more subtle and easier to apply.