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VIM-2 beta-lactamase in Pseudomonas aeruginosa isolates from Zagreb, Croatia
Scandinavian Journal of Infectious Diseases, 42 (2010), 3; 193-197. https://doi.org/10.3109/00365540903426582
Bošnjak, Zrinka; Bedenić, Branka; Mazzariol, Annarita; Jarža-Davila, Neda; Šuto, Sandra; Kalenić, Smilja

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Bošnjak, Z., Bedenić, B., Mazzariol, A., Jarža Davila, N., Šuto, S. i Kalenić, S. (2010). VIM-2 beta-lactamase in Pseudomonas aeruginosa isolates from Zagreb, Croatia. Scandinavian Journal of Infectious Diseases, 42. (3), 193-197. doi: 10.3109/00365540903426582

Bošnjak, Zrinka, et al. "VIM-2 beta-lactamase in Pseudomonas aeruginosa isolates from Zagreb, Croatia." Scandinavian Journal of Infectious Diseases, vol. 42, br. 3, 2010, str. 193-197. https://doi.org/10.3109/00365540903426582

Bošnjak, Zrinka, Branka Bedenić, Annarita Mazzariol, Neda Jarža Davila, Sandra Šuto i Smilja Kalenić. "VIM-2 beta-lactamase in Pseudomonas aeruginosa isolates from Zagreb, Croatia." Scandinavian Journal of Infectious Diseases 42, br. 3 (2010): 193-197. https://doi.org/10.3109/00365540903426582

Bošnjak, Z., et al. (2010) 'VIM-2 beta-lactamase in Pseudomonas aeruginosa isolates from Zagreb, Croatia', Scandinavian Journal of Infectious Diseases, 42(3), str. 193-197. doi: 10.3109/00365540903426582

Bošnjak Z, Bedenić B, Mazzariol A, Jarža Davila N, Šuto S, Kalenić S. VIM-2 beta-lactamase in Pseudomonas aeruginosa isolates from Zagreb, Croatia. Scandinavian Journal of Infectious Diseases [Internet]. 03.2010. [pristupljeno 09.05.2024.];42(3):193-197. doi: 10.3109/00365540903426582

Z. Bošnjak, B. Bedenić, A. Mazzariol, N. Jarža Davila, S. Šuto i S. Kalenić, "VIM-2 beta-lactamase in Pseudomonas aeruginosa isolates from Zagreb, Croatia", Scandinavian Journal of Infectious Diseases, vol. 42, br. 3, str. 193-197, Ožujak 2010. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:105:875221. [Citirano: 09.05.2024.]

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Naslov (engleski)VIM-2 beta-lactamase in Pseudomonas aeruginosa isolates from Zagreb, Croatia
AutorZrinka Bošnjak
AutorBranka Bedenić
AutorAnnarita Mazzariol
AutorNeda Jarža-Davila
AutorSandra Šuto
AutorSmilja Kalenić
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Kliničke medicinske znanosti
Sažetak (engleski)
The aim of this investigation was to characterize metallo-beta-lactamases (MBLs) in Pseudomonas aeruginosa isolates from Zagreb, Croatia. One hundred P. aeruginosa isolates with reduced susceptibility to either imipenem or meropenem were tested for the production of MBLs by MBL-Etest. The susceptibility to a wide range of antibiotics was determined by broth microdilution method. The presence of bla(MBL) genes was detected by polymerase chain reaction (PCR). Hydrolysis of 0.1 mM imipenem by crude enzyme preparations of beta-lactamases was monitored by UV spectrophotometer. Outer membrane proteins were prepared and analysed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Six out of 100 isolates were positive for MBLs by Etest. All strains were resistant to gentamicin, ceftazidime and cefotaxime, and all except 1 were resistant to imipenem. Six strains positive for MBLs by Etest were identified as VIM MBL-producers by PCR. Sequencing of bla(VIM) genes revealed the production of VIM-2 beta-lactamase in all 6 strains. This investigation proved the occurrence of VIM-2 beta-lactamase among P. aeruginosa strains from Zagreb, Croatia. VIM-2 beta-lactamase with similar properties has previously been described in another region of Croatia and in Italy, France, Spain, Greece, Taiwan and South Korea, suggesting that this type of enzyme is widespread in the Mediterranean region of Europe and in the Far East.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran
Verzija publikacijeZavršna verzija rukopisa prihvaćena za objavljivanje (postprint)
Naslov časopisaScandinavian Journal of Infectious Diseases
Brojčani podacivol. 42, br. 3, str. 193-197
p-ISSN0036-5548
DOIhttps://doi.org/10.3109/00365540903426582
URN:NBNurn:nbn:hr:105:875221
Datum objave publikacije2010-03
URL dokumentahttps://doi.org/10.3109/00365540903426582
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Zaštićeno autorskim pravom.
Datum i vrijeme pohrane2024-03-27 19:07:19