prikaz prve stranice dokumenta Global DNA methylation and chondrogenesis of rat limb buds in a three-dimensional organ culture system
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Global DNA methylation and chondrogenesis of rat limb buds in a three-dimensional organ culture system
Bosnian Journal of Basic Medical Sciences, 22 (2022), 4; 560-568. https://doi.org/10.17305/bjbms.2021.6584
Mužić Radović, Vedrana; Bunoza, Paula; Marić, Tihana; Himelreich-Perić, Marta; Bulić-Jakuš, Floriana; Takahashi, Marta; Jurić-Lekić, Gordana; Sinčić, Nino; Ježek, Davor; Katušić-Bojanac, Ana

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Mužić Radović, V., Bunoza, P., Marić, T., Himelreich Perić, M., Bulić Jakuš, F., Takahashi, M. ... Katušić Bojanac, A. (2022). Global DNA methylation and chondrogenesis of rat limb buds in a three-dimensional organ culture system. Bosnian Journal of Basic Medical Sciences, 22. (4), 560-568. doi: 10.17305/bjbms.2021.6584

Mužić Radović, Vedrana, et al. "Global DNA methylation and chondrogenesis of rat limb buds in a three-dimensional organ culture system." Bosnian Journal of Basic Medical Sciences, vol. 22, br. 4, 2022, str. 560-568. https://doi.org/10.17305/bjbms.2021.6584

Mužić Radović, Vedrana, Paula Bunoza, Tihana Marić, Marta Himelreich Perić, Floriana Bulić Jakuš, Marta Takahashi, Gordana Jurić Lekić, Nino Sinčić, Davor Ježek i Ana Katušić Bojanac. "Global DNA methylation and chondrogenesis of rat limb buds in a three-dimensional organ culture system." Bosnian Journal of Basic Medical Sciences 22, br. 4 (2022): 560-568. https://doi.org/10.17305/bjbms.2021.6584

Mužić Radović, V., et al. (2022) 'Global DNA methylation and chondrogenesis of rat limb buds in a three-dimensional organ culture system', Bosnian Journal of Basic Medical Sciences, 22(4), str. 560-568. doi: 10.17305/bjbms.2021.6584

Mužić Radović V, Bunoza P, Marić T, Himelreich Perić M, Bulić Jakuš F, Takahashi M, i sur.. Global DNA methylation and chondrogenesis of rat limb buds in a three-dimensional organ culture system. Bosnian Journal of Basic Medical Sciences [Internet]. 29.06.2022. [pristupljeno 15.03.2025.];22(4):560-568. doi: 10.17305/bjbms.2021.6584

V. Mužić Radović, et al., "Global DNA methylation and chondrogenesis of rat limb buds in a three-dimensional organ culture system", Bosnian Journal of Basic Medical Sciences, vol. 22, br. 4, str. 560-568, Lipanj 2022. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:105:189453. [Citirano: 15.03.2025.]

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Podaci o radu
Naslov (engleski)Global DNA methylation and chondrogenesis of rat limb buds in a three-dimensional organ culture system
AutorVedrana Mužić Radović
AutorPaula Bunoza
AutorTihana Marić
AutorMarta Himelreich-Perić
AutorFloriana Bulić-Jakuš
AutorMarta Takahashi
AutorGordana Jurić-Lekić
AutorNino Sinčić
AutorDavor Ježek
AutorAna Katušić-Bojanac
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
(KATEDRA ZA HISTOLOGIJU I EMBRIOLOGIJU)
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
(KATEDRA ZA MEDICINSKU BIOLOGIJU)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Temeljne medicinske znanosti
Citologija, histologija i embriologija
Sažetak (engleski)
Although DNA methylation epigenetically regulates development, data on global DNA methylation during development of limb buds (LBs) are scarce. We aimed to investigate the global DNA methylation developmental dynamics in rat LBs cultivated in a serum-supplemented (SS) and in chemically defined serum- and protein-free (SF) three-dimensional organ culture. Fischer rat front- and hind-LBs at 13th and 14th gestation days (GD) were cultivated at the air-liquid interface in Eagle's Minimal Essential... Više Medium (MEM) or MEM with 50% rat serum for 14 days, as SF and SS conditions, respectively. The methylation of repetitive DNA sequences (SINE rat ID elements) was assessed by pyrosequencing. Development was evaluated by light microscopy and extracellular matrix glycosaminoglycans staining by Safranin O. Upon isolation, weak Safranin O staining was present only in more developed GD14 front-LBs. Chondrogenesis proceeded well in all cultures towards day 14, except in the SF-cultivated GD13 hind-LBs, where Safranin O staining was almost absent on day 3. That was associated with a higher percentage of DNA methylation than in SF-cultivated GD13 front-LBs on day three. In SF-cultivated front-LBs, a significant methylation increase between the 3rd and 14th day was detected. In SS-cultivated GD13 front-LBs, methylation increased significantly on day three and then decreased. In older GD14 SS-cultivated LBs, there was no increase of DNA methylation, but they were significantly hypomethylated relative to the SS-cultivated GD13 at days 3 and 14. We confirmed that the global DNA methylation increase is associated with less developed limb organ primordia that strive towards differentiation in vitro, which is of importance for regenerative medicine strategies. Sakrij dio sažetka
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaBosnian Journal of Basic Medical Sciences
Brojčani podacivol. 22, br. 4, str. 560-568
p-ISSN1512-8601
e-ISSN1840-4812
DOIhttps://doi.org/10.17305/bjbms.2021.6584
URN:NBNurn:nbn:hr:105:189453
Datum objave publikacije2022-06-29
URL dokumentahttps://www.bjbms.org/ojs/index.php/bjbms/article/view/6584
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje 4.0 međunarodna (CC BY 4.0)
Datum i vrijeme pohrane2022-10-19 11:10:42
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