prikaz prve stranice dokumenta Extended Prophylactic Effect of N-tert-Butyl-α-phenylnitron against Oxidative/Nitrosative Damage Caused by the DNA-Hypomethylating Drug 5-Azacytidine in the Rat Placenta
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Extended Prophylactic Effect of N-tert-Butyl-α-phenylnitron against Oxidative/Nitrosative Damage Caused by the DNA-Hypomethylating Drug 5-Azacytidine in the Rat Placenta
International Journal of Molecular Sciences, 23 (2022), 2; 603. https://doi.org/10.3390/ijms23020603
Sobočan, Nikola; Himelreich-Perić, Marta; Katušić-Bojanac, Ana; Krasić, Jure; Sinčić, Nino; Majić, Željka; Jurić-Lekić, Gordana; Šerman, Ljiljana; Marić, Andreja; Ježek, Davor; Bulić-Jakuš, Floriana

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Sobočan, N., Himelreich Perić, M., Katušić Bojanac, A., Krasić, J., Sinčić, N., Majić, Ž. ... Bulić Jakuš, F. (2022). Extended Prophylactic Effect of N-tert-Butyl-α-phenylnitron against Oxidative/Nitrosative Damage Caused by the DNA-Hypomethylating Drug 5-Azacytidine in the Rat Placenta. International Journal of Molecular Sciences, 23. (2). doi: 10.3390/ijms23020603

Sobočan, Nikola, et al. "Extended Prophylactic Effect of N-tert-Butyl-α-phenylnitron against Oxidative/Nitrosative Damage Caused by the DNA-Hypomethylating Drug 5-Azacytidine in the Rat Placenta." International Journal of Molecular Sciences, vol. 23, br. 2, 2022. https://doi.org/10.3390/ijms23020603

Sobočan, Nikola, Marta Himelreich Perić, Ana Katušić Bojanac, Jure Krasić, Nino Sinčić, Željka Majić, Gordana Jurić Lekić, et al. "Extended Prophylactic Effect of N-tert-Butyl-α-phenylnitron against Oxidative/Nitrosative Damage Caused by the DNA-Hypomethylating Drug 5-Azacytidine in the Rat Placenta." International Journal of Molecular Sciences 23, br. 2 (2022). https://doi.org/10.3390/ijms23020603

Sobočan, N., et al. (2022) 'Extended Prophylactic Effect of N-tert-Butyl-α-phenylnitron against Oxidative/Nitrosative Damage Caused by the DNA-Hypomethylating Drug 5-Azacytidine in the Rat Placenta', International Journal of Molecular Sciences, 23(2). doi: 10.3390/ijms23020603

Sobočan N, Himelreich Perić M, Katušić Bojanac A, Krasić J, Sinčić N, Majić Ž, i sur.. Extended Prophylactic Effect of N-tert-Butyl-α-phenylnitron against Oxidative/Nitrosative Damage Caused by the DNA-Hypomethylating Drug 5-Azacytidine in the Rat Placenta. International Journal of Molecular Sciences [Internet]. 06.01.2022. [pristupljeno 17.04.2024.];23(2). doi: 10.3390/ijms23020603

N. Sobočan, et al., "Extended Prophylactic Effect of N-tert-Butyl-α-phenylnitron against Oxidative/Nitrosative Damage Caused by the DNA-Hypomethylating Drug 5-Azacytidine in the Rat Placenta", International Journal of Molecular Sciences, vol. 23, br. 2, Siječanj 2022. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:105:168102. [Citirano: 17.04.2024.]

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Naslov (engleski)Extended Prophylactic Effect of N-tert-Butyl-α-phenylnitron against Oxidative/Nitrosative Damage Caused by the DNA-Hypomethylating Drug 5-Azacytidine in the Rat Placenta
AutorNikola Sobočan
AutorMarta Himelreich-Perić
AutorAna Katušić-Bojanac
AutorJure Krasić
AutorNino Sinčić
AutorŽeljka Majić
AutorGordana Jurić-Lekić
AutorLjiljana Šerman
AutorAndreja Marić
AutorDavor Ježek
AutorFloriana Bulić-Jakuš
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
(KATEDRA ZA HISTOLOGIJU I EMBRIOLOGIJU)
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
(KATEDRA ZA MEDICINSKU BIOLOGIJU)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Temeljne medicinske znanosti
Citologija, histologija i embriologija
Sažetak (engleski)
Antioxidant N-tert-Butyl-α-phenylnitron (PBN) partly protected embryos from the negative effects of a DNA demethylating drug 5-azacytidine during pregnancy. Our aim was to investigate PBN’s impact on the placenta. Fischer rat dams were treated on gestation days (GD) 12 and 13 by PBN (40 mg/kg), followed by 5azaC (5 mg/kg) after one hour. Global methylation was assessed by pyrosequencing. Numerical density was calculated from immunohistochemical expression in single cells for proliferating (PCNA), oxidative (oxoguanosine) and nitrosative (nitrotyrosine) activity. Results were compared with the PBN-treated and control rats. PBN-pretreatment significantly increased placental weight at GD15 and GD20, diminished by 5azaC, and diminished apoptosis in GD 20 placentas caused by 5azaC. Oxoguanosine expression in placentas of 5azaC-treated dams was especially high in the placental labyrinth on GD 15, while PBN-pretreatment lowered its expression on GD 15 and GD 20 in both the labyrinth and basal layer. 5azaC enhanced nitrotyrosine level in the labyrinth of both gestational stages, while PBN-pretreatment lowered it. We conclude that PBN exerted its prophylactic activity against DNA hypomethylating agent 5azaC in the placenta through free radical scavenging, especially in the labyrinthine part of the placenta until the last day of pregnancy.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaInternational Journal of Molecular Sciences
Brojčani podacivol. 23, br. 2, 603
e-ISSN1422-0067
DOIhttps://doi.org/10.3390/ijms23020603
URN:NBNurn:nbn:hr:105:168102
Datum objave publikacije2022-01-06
ProjektŠifra: KK.01.1.1.01.0008
Naziv (hrvatski): Reproduktivna i regenerativna medicina - istraživanje novih platformi i potencijala
Voditelj: Ana Katušić Bojanac
Pravna nadležnost: eu
Financijer: MZO
ProjektŠifra: 108-1080399-0335
Naziv (hrvatski): Eksperimentalni embrionalni tumori i razvoj zametaka sisavaca in vitro i in vivo
Naziv (engleski): Experimental embryonic tumors and mammalian development in vitro and in vivo
Voditelj: Florijana Jakuš
Pravna nadležnost: Hrvatska
Financijer: MZOS
Linija financiranja: ZP
URL dokumentahttps://www.mdpi.com/1422-0067/23/2/603
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje 4.0 međunarodna (CC BY 4.0)
Datum i vrijeme pohrane2022-11-15 09:31:55