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Transplantation of neural stem cells improves recovery of stroke-affected mice and induces cell-specific changes in GSDMD and MLKL expression
Frontiers in Molecular Neuroscience, 17 (2024); 1439994. https://doi.org/10.3389/fnmol.2024.1439994
Lisjak, Damir; Alić, Ivan; Šimunić, Iva; Mitrečić, Dinko

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Lisjak, D., Alić, I., Šimunić, I. i Mitrečić, D. (2024). Transplantation of neural stem cells improves recovery of stroke-affected mice and induces cell-specific changes in GSDMD and MLKL expression. Frontiers in Molecular Neuroscience, 17.. doi: 10.3389/fnmol.2024.1439994

Lisjak, Damir, et al. "Transplantation of neural stem cells improves recovery of stroke-affected mice and induces cell-specific changes in GSDMD and MLKL expression." Frontiers in Molecular Neuroscience, vol. 17, 2024. https://doi.org/10.3389/fnmol.2024.1439994

Lisjak, Damir, Ivan Alić, Iva Šimunić i Dinko Mitrečić. "Transplantation of neural stem cells improves recovery of stroke-affected mice and induces cell-specific changes in GSDMD and MLKL expression." Frontiers in Molecular Neuroscience 17 (2024). https://doi.org/10.3389/fnmol.2024.1439994

Lisjak, D., et al. (2024) 'Transplantation of neural stem cells improves recovery of stroke-affected mice and induces cell-specific changes in GSDMD and MLKL expression', Frontiers in Molecular Neuroscience, 17. doi: 10.3389/fnmol.2024.1439994

Lisjak D, Alić I, Šimunić I, Mitrečić D. Transplantation of neural stem cells improves recovery of stroke-affected mice and induces cell-specific changes in GSDMD and MLKL expression. Frontiers in Molecular Neuroscience [Internet]. 15.08.2024. [pristupljeno 04.04.2025.];17. doi: 10.3389/fnmol.2024.1439994

D. Lisjak, I. Alić, I. Šimunić i D. Mitrečić, "Transplantation of neural stem cells improves recovery of stroke-affected mice and induces cell-specific changes in GSDMD and MLKL expression", Frontiers in Molecular Neuroscience, vol. 17, Kolovoz 2024. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:105:100137. [Citirano: 04.04.2025.]

Za citiranje koristite ovu mrežnu adresu: https://urn.nsk.hr/urn:nbn:hr:105:100137
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Naslov (engleski)Transplantation of neural stem cells improves recovery of stroke-affected mice and induces cell-specific changes in GSDMD and MLKL expression
AutorDamir Lisjak
AutorIvan Alić
AutorIva Šimunić
AutorDinko Mitrečić
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
(HRVATSKI INSTITUT ZA ISTRAŽIVANJE MOZGA)
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
(KATEDRA ZA HISTOLOGIJU I EMBRIOLOGIJU)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Temeljne medicinske znanosti
Citologija, histologija i embriologija
Sažetak (engleski)
Introduction: Stroke, the second leading cause of death and disability in Europe, is primarily caused by interrupted blood supply, leading to ischemia-reperfusion (IR) injury and subsequent neuronal death. Current treatment options are limited, highlighting the need for novel therapies. Neural stem cells (NSCs) have shown promise in treating various neurological disorders, including stroke. However, the underlying mechanisms of NSC-mediated recovery remain unclear. Methods: Eighty... Više C57Bl/6-Tyrc-Brd mice underwent ischemic stroke induction and were divided into four groups: sham, stroke-affected, stroke-affected with basal cell medium injection, and stroke-affected with NSCs transplantation. NSCs, isolated from mouse embryos, were stereotaxically transplanted into the stroke-affected brains. Magnetic resonance imaging (MRI) and neurological scoring were used to assess recovery. Immunohistochemical analysis and gene expression assays were performed to evaluate pyroptosis and necroptosis markers. Results: NSC transplantation significantly improved neurological recovery compared to control groups. In addition, although not statistically significant, NSCs reduced stroke volume. Immunohistochemical analysis revealed upregulation of Gasdermin D (GSDMD) expression post-stroke, predominantly in microglia and astrocytes. However, NSC transplantation led to a reduction in GSDMD signal intensity in astrocytes, suggesting an effect of NSCs on GSDMD activity. Furthermore, NSCs downregulated Mixed Lineage Kinase Domain-Like Protein (Mlkl) expression, indicating a reduction in necroptosis. Immunohistochemistry demonstrated decreased phosphorylated MLKL (pMLKL) signal intensity in neurons while stayed the same in astrocytes following NSC transplantation, along with increased distribution in microglia. Discussion: NSC transplantation holds therapeutic potential in stroke recovery by targeting pyroptosis and necroptosis pathways. These findings shed light on the mechanisms underlying NSC-mediated neuroprotection and support their further exploration as a promising therapy for stroke patients. Sakrij dio sažetka
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaFrontiers in Molecular Neuroscience
Brojčani podacivol. 17, 1439994
e-ISSN1662-5099
DOIhttps://doi.org/10.3389/fnmol.2024.1439994
URN:NBNurn:nbn:hr:105:100137
Datum objave publikacije2024-08-15
ProjektŠifra: IP-2016-06-9451
Naziv (hrvatski): Matične stanice usne šupljine čovjeka za liječenje ishemijske bolesti mozga
Naziv (engleski): Stem cells from human oral mucosa for treatment of brain ischemia
Kratica: ORASTEM
Voditelj: Dinko Mitrečić
Pravna nadležnost: Hrvatska
Financijer: Hrvatska zaklada za znanost
Linija financiranja: Research Projects
ProjektŠifra: IP-2022-10-4656
Naziv (hrvatski): Otkrivanje neurorazvojne osnove Downovog sindroma pomoću jedinstvenih organoida ljudskog mozga
Naziv (engleski): Revealing the Neurodevelopmental Basis of Down Syndrome on a Unique Human Brain Organoid Model
Kratica: DevDown
Voditelj: Dinko Mitrečić
Pravna nadležnost: Hrvatska
Financijer: Hrvatska zaklada za znanost
Linija financiranja: Research Projects
ProjektŠifra: KK.01.1.1.01.0007
Naziv (hrvatski): Znanstveni centar izvrsnosti - eksperimentalna i klinička istraživanja hipoksijsko-ishemijskog oštećenja mozga u perinatalnoj i odrasloj dobi
Naziv (engleski): Scientific center of excellence - Experimental and clinical research of hypoxic-ischemic brain damage in perinatal and adulthood
Kratica: ZCI-NEURO
Voditelj: Miloš Judaš
Pravna nadležnost: eu
Financijer: Europska unija
Linija financiranja: European Regional Development Fund
URL dokumentahttps://www.frontiersin.org/articles/10.3389/fnmol.2024.1439994/full
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje 4.0 međunarodna (CC BY 4.0)
Datum i vrijeme pohrane2025-03-24 09:42:25
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