Optimization of fermentation processes requires monitoring the species composition of starter cultures and their growth during fermentation. Most starter cultures contain closely related species. Nowadays, high-resolution melting (HRM) analysis is extensively used for multiplex identification of closely related species. In the present paper, we applied real-time polymerase chain reaction (PCR) with HRM analysis for the detection and differentiation of Lactobacillus sakei and L. curvatus. A primer pair was selected for the site of the rpoA gene of Lactobacillus spp. Eleven starter cultures and fifteen fermented sausages with a known bacterial composition were successfully tested using real-time PCR with HRM analysis with the developed primer pair.; Za optimiranje procesa potrebno je pratiti sastav starter kulture i njezin rast tijekom fermentacije. Većina starter kultura sadržava srodne mikroorganizme. U današnje se vrijeme za identifikaciju većeg broja srodnih vrsta često upotrebljava postupak analize krivulje mekšanja DNK visoke razlučivosti. Stoga je u radu za detekciju i razlikovanje bakterija Lactobacillus sakei i L. curvatus upotrijebljena lančana reakcija polimeraze (PCR) u stvarnom vremenu, te je provedena analiza krivulje mekšanja DNK visoke razlučivosti. Odabran je par početnica za ciljano mjesto vezivanja u genu rpoA, izoliranom iz bakterija roda Lactobacillus spp. S tim je parom početnica uspješno ispitano 1 1 starter kultura i 15 uzoraka fermentiranih kobasica poznatog sastava bakterija.