The purpose of this paper is to point out the connection between the number of entrepreneurial centers and the development of entrepreneurs in the Republic of Croatia. Development of entrepreneurship centers is important for entrepreneurs, local communities, counties and the country itself. Entrepreneurs are the carriers of economic development who manage to initiate change regardless of major challenges and risks.The subject of this work are entrepreneurship centers, and the paper explains the terms of entrepreneurship, entrepreneurial infrastructure and entrepreneurship centers.The role of entrepreneurship centers is focused on encouraging and promoting entrepreneurship, providing advisory assistance, informing about possibilities to enter entrepreneurship, informing about project financing possibilities, providing marketing services, organizing seminars, cooperating with local government in promoting entrepreneurship, internet services and connecting entrepreneurs.As an example of an entrepreneurial center in the Republic of Croatia, the Zovnica Entrepreneurial Center from the town of Imotski was presented.