Two spotted spider mite (Tetranychus urticae Koch) is one of the most economically significant pests that feeds on many species of cultivated and wild plants. Its development thrives in dry and warm weather, and in a very short time it can cause great damage, which manifests in the plants by characteristic drying and shedding of leaves. The control of two spotted spider mites today is mainly based on the application of acaricides, and due to the short life cycle and a large number of generations and a large number of treatments, this pest quickly develops resistance. As a consequence of the resistance development, controling the mite has become problematic in many areas around the world. Therefore, it's necessary to improve existing and or develop new, alternative, non-chemical control measures that will be sufficiently effective to prevent biodiversity loss and significant yield loss. The aim of this thesis is to present non-pesticide control measures (agrotechnical, mechanical, physical), biopesticides (predators, microbiological combinations, plant extracts and essential oils) and biotechnical agents that can be used in the control of two spotted spider mite; show examples of application and their effectiveness and determine the appropriate application of certain non-pesticide control measures in practice.