Corn is with rice and wheat the leading agricultural culture in the world. Practically every part of the corn plant can be used in a variety of ways and for different purposes. Corn is a source of food for humans and animals. In the production of biofuels, corn can be used for the production of liquid (ethanol) and solid biofuels (pellets / briquettes). Ethanol can be obtained from corn grain but also from corn stover, which remains on the field after harvest of corn. Corn stover represents an important source of organic matter and has an important influence on the biological, chemical and physical properties of the soil and should not be regarded as waste. Biomass of agricultural origin, as well as corn stover, is highly acceptable fuel from the standpoint of environmental impact. The aim of this graduate thesis is to determine the possibilities of collecting corn stover from a certain area at different plant population densities of corn hybrid P9903 with machinery used on the family farm for collecting aboveground mass in the field. Collection was performed with round baler with pick-up device in three different scenarios: (I) baling, (II) collecting in windrows + baling and (III) mulching + collection in windrows + baling. Corn stover yield was in the range of 12,389 to 13,118 kg / ha. Collection efficiency was from 49 to 78% depending about collection scenarios.