prikaz prve stranice dokumenta Biophysical insights from a single chain camelid antibody directed against the Disrupted-in-Schizophrenia 1 protein
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Biophysical insights from a single chain camelid antibody directed against the Disrupted-in-Schizophrenia 1 protein
PLoS ONE, 13 (2018), e0191162; 18. https://doi.org/10.1371/journal.pone.0191162
Yerabham, Antony S. K.; Müller-Schiffmann, Andreas; Ziehm, Tamar; Stadler, Andreas; Köber, Sabrina; Indurkhya, Xela; Marreiros, Rita; Trossbach, Svenja V.; Bradshaw, Nicholas J.; Prikulis, Ingrid; Willbold, Dieter; Weiergräber, Oliver H.; Korth, Carsten

Citirajte ovaj rad

Yerabham, A. S. K., Müller Schiffmann, A., Ziehm, T., Stadler, A., Köber, S., Indurkhya, X. ... Korth, C. (2018). Biophysical insights from a single chain camelid antibody directed against the Disrupted-in-Schizophrenia 1 protein. PLoS ONE, 13. (e0191162). doi: 10.1371/journal.pone.0191162

Yerabham, Antony S. K., et al. "Biophysical insights from a single chain camelid antibody directed against the Disrupted-in-Schizophrenia 1 protein." PLoS ONE, vol. 13, br. e0191162, 2018. https://doi.org/10.1371/journal.pone.0191162

Yerabham, Antony S. K., Andreas Müller Schiffmann, Tamar Ziehm, Andreas Stadler, Sabrina Köber, Xela Indurkhya, Rita Marreiros, et al. "Biophysical insights from a single chain camelid antibody directed against the Disrupted-in-Schizophrenia 1 protein." PLoS ONE 13, br. e0191162 (2018). https://doi.org/10.1371/journal.pone.0191162

Yerabham, A. S. K., et al. (2018) 'Biophysical insights from a single chain camelid antibody directed against the Disrupted-in-Schizophrenia 1 protein', PLoS ONE, 13(e0191162). doi: 10.1371/journal.pone.0191162

Yerabham ASK, Müller Schiffmann A, Ziehm T, Stadler A, Köber S, Indurkhya X, i sur.. Biophysical insights from a single chain camelid antibody directed against the Disrupted-in-Schizophrenia 1 protein. PLoS ONE [Internet]. 11.01.2018. [pristupljeno 25.04.2024.];13(e0191162). doi: 10.1371/journal.pone.0191162

A. S. K. Yerabham, et al., "Biophysical insights from a single chain camelid antibody directed against the Disrupted-in-Schizophrenia 1 protein", PLoS ONE, vol. 13, br. e0191162, Siječanj 2018. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:193:359049. [Citirano: 25.04.2024.]

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Naslov (engleski)Biophysical insights from a single chain camelid antibody directed against the Disrupted-in-Schizophrenia 1 protein
AutorAntony S. K. Yerabham
AutorAndreas Müller-Schiffmann
AutorTamar Ziehm
AutorAndreas Stadler
AutorSabrina Köber
AutorXela Indurkhya
AutorRita Marreiros
AutorSvenja V. Trossbach
AutorNicholas J. Bradshaw
AutorIngrid Prikulis
AutorDieter Willbold
AutorOliver H. Weiergräber
AutorCarsten Korth
Autorova ustanovaSveučilište u Rijeci
(Fakultet biotehnologije i razvoja lijekova)
Znanstveno / umjetničko
područje, polje i grana		BIOTEHNIČKE ZNANOSTI
Biotehnologija
Biologija
Sažetak (engleski)
Accumulating evidence suggests an important role for the Disrupted-in-Schizophrenia 1 (DISC1) protein in neurodevelopment and chronic mental illness. In particular, the C-terminal 300 amino acids of DISC1 have been found to mediate important protein-protein interactions and to harbor functionally important phosphorylation sites and disease-associated polymorphisms. However, long disordered regions and oligomer-forming subdomains have so far impeded structural analysis. VHH domains derived from camelid heavy chain only antibodies are minimal antigen binding modules with appreciable solubility and stability, which makes them well suited for the stabilizing proteins prior to structural investigation. Here, we report on the generation of a VHH domain derived from an immunized Lama glama, displaying high affinity for the human DISC1 C region (aa 691±836), and its characterization by surface plasmon resonance, size exclusion chromatography and immunological techniques. The VHH-DISC1 (C region) complex was also used for structural investigation by small angle X-ray scattering analysis. In combination with molecular modeling, these data support predictions regarding the three-dimensional fold of this DISC1 segment as well as its steric arrangement in complex with our VHH antibody.
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran - međunarodna recenzija
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaPLoS ONE
Brojčani podacivol. 13, br. e0191162, 18
e-ISSN1932-6203
DOIhttps://doi.org/10.1371/journal.pone.0191162
URN:NBNurn:nbn:hr:193:359049
Datum objave publikacije2018-01-11
URL dokumentahttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0191162
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje 4.0 međunarodna (CC BY 4.0)
Datum i vrijeme pohrane2018-09-14 11:48:51