Foundation of this reasearch explores the perception of female preschool teachers about the experience of working with male preschool teachers, the well-being of male educators within the profession and the presence of the "male role model". The early childhood care and education profession is trying to look from a gender perspective, or to gain insight into the state of gender balance in early and preschool upbringing and education. During April and May 2018 a survey was carried out on the areas of Kastav, Matulji, Opatija and Rijeka and included kindergartens:"Vladimir Nazor", the private kindergarten "Poneštrica", "Matulji" kindergarten, the kindergarten "Opatija" as well as the kindergarten "Rijeka", ie centers of preschool education "Zamet" and "Maestral". The population of the respondents are female preschool teachers, and the size of the target sample is 160. The results of the research showed that almost half of the female preschool teachers did not have the experience of working with male preschool teacheres and that men were still under-represented in early and pre-school upbringing and education. Female preschool teachers who have worked with male preschool teachers would recommend cooperation with them. However, as female preschool teachers favor the traditional division of jobs within the early chidhood care and education, their attitude towards cooperation with male preschool teacher is more negative. On the other hand, female preschool teachers who do not have the experience of working with a male preschool teachers would also recommend co-operation, which suggests that experience is not crucial in forming attidudes about co-operation.