prikaz prve stranice dokumenta Optimization of an ex vivo gene transfer to the hamstrings tendons muscle remnants: potential for genetic enhancement of bone healing
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Optimization of an ex vivo gene transfer to the hamstrings tendons muscle remnants: potential for genetic enhancement of bone healing
Croatian Medical Journal, 60 (2019), 3; 201-211. https://doi.org/10.3325/cmj.2019.60.201
Rod, Eduard; Matić, Igor; Antunović, Maja; Vetma, Vesna; Pavičić, Ivan; Hudetz, Damir; Marijanović, Inga; Primorac, Dragan; Ivković, Alan

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Rod, E., Matić, I., Antunović, M., Vetma, V., Pavičić, I., Hudetz, D. ... Ivković, A. (2019). Optimization of an ex vivo gene transfer to the hamstrings tendons muscle remnants: potential for genetic enhancement of bone healing. Croatian Medical Journal, 60. (3), 201-211. doi: 10.3325/cmj.2019.60.201

Rod, Eduard, et al. "Optimization of an ex vivo gene transfer to the hamstrings tendons muscle remnants: potential for genetic enhancement of bone healing." Croatian Medical Journal, vol. 60, br. 3, 2019, str. 201-211. https://doi.org/10.3325/cmj.2019.60.201

Rod, Eduard, Igor Matić, Maja Antunović, Vesna Vetma, Ivan Pavičić, Damir Hudetz, Inga Marijanović, Dragan Primorac i Alan Ivković. "Optimization of an ex vivo gene transfer to the hamstrings tendons muscle remnants: potential for genetic enhancement of bone healing." Croatian Medical Journal 60, br. 3 (2019): 201-211. https://doi.org/10.3325/cmj.2019.60.201

Rod, E., et al. (2019) 'Optimization of an ex vivo gene transfer to the hamstrings tendons muscle remnants: potential for genetic enhancement of bone healing', Croatian Medical Journal, 60(3), str. 201-211. doi: 10.3325/cmj.2019.60.201

Rod E, Matić I, Antunović M, Vetma V, Pavičić I, Hudetz D, i sur.. Optimization of an ex vivo gene transfer to the hamstrings tendons muscle remnants: potential for genetic enhancement of bone healing. Croatian Medical Journal [Internet]. 2019. [pristupljeno 16.04.2024.];60(3):201-211. doi: 10.3325/cmj.2019.60.201

E. Rod, et al., "Optimization of an ex vivo gene transfer to the hamstrings tendons muscle remnants: potential for genetic enhancement of bone healing", Croatian Medical Journal, vol. 60, br. 3, str. 201-211, 2019. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:184:722027. [Citirano: 16.04.2024.]

Za citiranje koristite ovu mrežnu adresu: https://urn.nsk.hr/urn:nbn:hr:184:722027
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Podaci o radu
Naslov (engleski)Optimization of an ex vivo gene transfer to the hamstrings tendons muscle remnants: potential for genetic enhancement of bone healing
AutorEduard Rod
AutorIgor Matić
AutorMaja Antunović
AutorVesna Vetma
AutorIvan Pavičić
AutorDamir Hudetz
AutorInga Marijanović
AutorDragan Primorac
AutorAlan Ivković
Autorova ustanovaSveučilište u Rijeci
Medicinski fakultet
(Katedra za medicinsku biologiju i genetiku)
Znanstveno / umjetničko
područje, polje i grana		PRIRODNE ZNANOSTI
Biologija
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Kliničke medicinske znanosti
Znanstveno / umjetničko
područje, polje i grana		INTERDISCIPLINARNA PODRUČJA ZNANOSTI
Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)
Sažetak (engleski)
Aim To assess whether an adenoviral vector carrying the bone morphogenetic protein genes (Ad.BMP-2) can transduce human muscle tissue and direct it toward osteogenic differentiation within one hour. Methods This in vitro study, performed at the Department of Molecular Biology, Faculty of Science, Zagreb from 2012 to 2017, used human muscle tissue samples collected during anterior cruciate ligament reconstructions performed in St Catherine Hospital, Zabok. Samples from 28 patients were transduced with adenoviral vector carrying firefly luciferase cDNA (Ad.luc) by using different doses and times of transduction, and with addition of positive ions for transduction enhancement. The optimized protocol was further tested on muscle samples from three new patients, which were transduced with Ad.BMP-2. Released bone morphogenetic protein 2 (BMP-2) levels in osteogenic medium were measured every three days during a period of 21 days. Expression of osteogenic markers was measured at day 14 and 21. After 21 days of cultivation, muscle tissue was immunohistochemically stained for collagen type I detection (COL-I). Results The new transduction protocol was established using 108 plaque-forming units (P < 0.001) as an optimal dose of adenoviral vector and 30 minutes (P < 0.001) as an optimal contact time. Positive ions did not enhance transduction. Samples transduced with Ad.BMP-2 according to the optimized protocol showed enhanced expression of osteogenic markers (P < 0.050), BMP-2 (P < 0.001), and COL I. Conclusion This study confirms that Ad.BMP-2 can transduce human muscle tissue and direct it toward osteogenic differentiation within 30 minutes.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaCroatian Medical Journal
Brojčani podacivol. 60, br. 3, str. 201-211
p-ISSN0353-9504
e-ISSN1332-8166
DOIhttps://doi.org/10.3325/cmj.2019.60.201
URN:NBNurn:nbn:hr:184:722027
Datum objave publikacije2019
URL dokumentahttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6563169/
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje-Nekomercijalno-Bez prerada 4.0 međunarodna (CC BY-NC-ND 4.0)
Datum i vrijeme pohrane2020-03-30 06:34:27