Orthodontic appliances have been used for decades as a primary way of treating orthodontic problems and achieving an aesthetically pleasing look. They are usually made of metal, which can easily corrode and release metal ions into saliva. Metal ions are known for their numerous negative biological effects, so a question, whether ions released by appliances cause damage to patients, rises. The goal of this paper was to test cytotoxic, genotoxic and prooxidative effects of metal ions released by appliance parts into saliva. The results show that a certain amount of those effects on cells is visible, but that it is not worrying to the point of saying that orthodontic appliances are harmful for human health. Cytotoxicity was measured using NR method, prooxidative effects were tasted using the DCFH-DA method and φX-174 RF I plasmid linearization, while genotoxicity was measured with comet assay. The eluation time has an impact on the composition of the saliva and biological effects can be measured. Most of the tested samples had a toxic effect on CAL 27 cell line. Regarding AGS cell line, lower concentrations of samples were shown to have a prooxidative effect, while higher concentrations had an antioxidative effect. The prooxidative effect tested using the φX-174 RF I plasmid linearization was weak. Metals present in the eluates do not cause permanent changes of the genetic material and their application is safe.