The aim of this study was to determine whether the white eye mutation in fruit flies Drosophila melanogaster is due to inheritance sex linked gene or it is autosomal mutation. We inoculate 5 males and 5 virgin females in special containers which we provide with recently prepaired base. After crossing the wild type flies (red eyes) and white flies through several generations we counted offsprings and confirm autosomal mutation as we realise 4 different colours of eye white, brown, red and scarlet. Red colour eyes are result of deposition two pigments xanthommatin (brown) and drosopterins (scarlet) in the pigment granules. W, st and bw genes encode ABC transporter protein that is responsible for transporting both pigments brown and scarlet. Mutation of both genes, st and bw, which encode defective protein causes white eyes due to ineffective transport of pigments in the pigment granules within the cell ommatidia. Null mutants of st have reduced capability to take up tryptophan and the result are scarlet eyes. Similarly, mutants with null allel at bw have reduced capability to take up guanine and the result are brown eyes. After counting the offspring of F2 generation the results weren´t similar to expected ratio 9:3:3:1. The reasons for mismatch with the ratio were possible errors in work such as replacement brown eye and scarlet eye flies, commpetition for food, blindness of white eye flies, using non-virgin flies and dependence on temperature of pigment production. Despite determination of mutation in this work we carried out crossing in which we got homozygotes of brown and scarlet flies.