Antiseptics are antimicrobial substances applied to skin and mucous membranes, so they are often called topical or local anti-infective or antimicrobial agents. They may be bacteriostatic or bactericidal, fungicides and virucides. They are used to prevent and treat various infective oral diseases, but unlike antibiotics they are not selective enough and cannot be absorbed because they could damage host’s cells and tissue. The importance of their use in dentistry was confirmed when it was proven that bacterial plaque is the main etiological factor in the formation of dental caries, as well as in pulp infections and periodontal diseases. Antiseptics are indispensable in preventive and therapeutic procedures in pediatric dentistry. These methods relate to a chemical plaque control and elimination of pathogenic microorganisms, and thus help in the prevention of caries and gingivitis. Furthermore, they are applied in endodontics such as vital and devitalizing pulpotomy and pulpectomy. Antiseptics can be applied in dentistry, in appropriate concentration, to oral mucosa, teeth surface and into the teeth in order to reduce the number of microorganisms. The success of the treatment depends on the correct choice of the antiseptic, which requires the knowledge of the antimicrobial spectrum and indications for adequate use of each antiseptic. The success also depends on its performance, i.e. on using the appropriate concentration and appropriate time of application of the antiseptic on the treated area (3).