prikaz prve stranice dokumenta Plasma Membrane Calcium ATPase-Neuroplastin Complexes Are Selectively Stabilized in GM1-Containing Lipid Rafts
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Plasma Membrane Calcium ATPase-Neuroplastin Complexes Are Selectively Stabilized in GM1-Containing Lipid Rafts
International Journal of Molecular Sciences, 22 (2021), 24; 13590. https://doi.org/10.3390/ijms222413590
Ilić, Katarina; Lin, Xiao; Malci, Ayse; Stojanović, Mario; Puljko, Borna; Rožman, Marko; Vukelić, Željka; Heffer, Marija; Montag, Dirk; Schnaar, Ronald L.; Kalanj-Bognar, Svjetlana; Herrera-Molina, Rodrigo; Mlinac-Jerković, Kristina

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Ilić, K., Lin, X., Malci, A., Stojanović, M., Puljko, B., Rožman, M. ... Mlinac Jerković, K. (2021). Plasma Membrane Calcium ATPase-Neuroplastin Complexes Are Selectively Stabilized in GM1-Containing Lipid Rafts. International Journal of Molecular Sciences, 22. (24). doi: 10.3390/ijms222413590

Ilić, Katarina, et al. "Plasma Membrane Calcium ATPase-Neuroplastin Complexes Are Selectively Stabilized in GM1-Containing Lipid Rafts." International Journal of Molecular Sciences, vol. 22, br. 24, 2021. https://doi.org/10.3390/ijms222413590

Ilić, Katarina, Xiao Lin, Ayse Malci, Mario Stojanović, Borna Puljko, Marko Rožman, Željka Vukelić, et al. "Plasma Membrane Calcium ATPase-Neuroplastin Complexes Are Selectively Stabilized in GM1-Containing Lipid Rafts." International Journal of Molecular Sciences 22, br. 24 (2021). https://doi.org/10.3390/ijms222413590

Ilić, K., et al. (2021) 'Plasma Membrane Calcium ATPase-Neuroplastin Complexes Are Selectively Stabilized in GM1-Containing Lipid Rafts', International Journal of Molecular Sciences, 22(24). doi: 10.3390/ijms222413590

Ilić K, Lin X, Malci A, Stojanović M, Puljko B, Rožman M, i sur.. Plasma Membrane Calcium ATPase-Neuroplastin Complexes Are Selectively Stabilized in GM1-Containing Lipid Rafts. International Journal of Molecular Sciences [Internet]. 18.12.2021. [pristupljeno 14.04.2024.];22(24). doi: 10.3390/ijms222413590

K. Ilić, et al., "Plasma Membrane Calcium ATPase-Neuroplastin Complexes Are Selectively Stabilized in GM1-Containing Lipid Rafts", International Journal of Molecular Sciences, vol. 22, br. 24, Prosinac 2021. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:105:896617. [Citirano: 14.04.2024.]

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Naslov (engleski)Plasma Membrane Calcium ATPase-Neuroplastin Complexes Are Selectively Stabilized in GM1-Containing Lipid Rafts
AutorKatarina Ilić
AutorXiao Lin
AutorAyse Malci
AutorMario Stojanović
AutorBorna Puljko
AutorMarko Rožman
AutorŽeljka Vukelić
AutorMarija Heffer
AutorDirk Montag
AutorRonald L. Schnaar
AutorSvjetlana Kalanj-Bognar
AutorRodrigo Herrera-Molina
AutorKristina Mlinac-Jerković
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
(KATEDRA ZA MEDICINSKU KEMIJU, BIOKEMIJU I KLINIČKU KEMIJU)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Temeljne medicinske znanosti
Sažetak (engleski)
The recent identification of plasma membrane (Ca2+)-ATPase (PMCA)-Neuroplastin (Np) complexes has renewed attention on cell regulation of cytosolic calcium extrusion, which is of particular relevance in neurons. Here, we tested the hypothesis that PMCA-Neuroplastin complexes exist in specific ganglioside-containing rafts, which could affect calcium homeostasis. We analyzed the abundance of all four PMCA paralogs (PMCA1-4) and Neuroplastin isoforms (Np65 and Np55) in lipid rafts and bulk membrane fractions from GM2/GD2 synthase-deficient mouse brains. In these fractions, we found altered distribution of Np65/Np55 and selected PMCA isoforms, namely PMCA1 and 2. Cell surface staining and confocal microscopy identified GM1 as the main complex ganglioside co-localizing with Neuroplastin in cultured hippocampal neurons. Furthermore, blocking GM1 with a specific antibody resulted in delayed calcium restoration of electrically evoked calcium transients in the soma of hippocampal neurons. The content and composition of all ganglioside species were unchanged in Neuroplastin-deficient mouse brains. Therefore, we conclude that altered composition or disorganization of ganglioside-containing rafts results in changed regulation of calcium signals in neurons. We propose that GM1 could be a key sphingolipid for ensuring proper location of the PMCA-Neuroplastin complexes into rafts in order to participate in the regulation of neuronal calcium homeostasis.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaInternational Journal of Molecular Sciences
Brojčani podacivol. 22, br. 24, 13590
e-ISSN1422-0067
DOIhttps://doi.org/10.3390/ijms222413590
URN:NBNurn:nbn:hr:105:896617
Datum objave publikacije2021-12-18
ProjektŠifra: IP-2016-06-8636
Naziv (hrvatski): Molekularni biljezi vulnerabilnosti, adaptacije i plastičnosti neurona u akutnoj i kroničnoj ozljedi mozga
Kratica: NeuroReact
Voditelj: Svjetlana Kalanj-Bognar
Pravna nadležnost: Hrvatska
Financijer: HRZZ
Linija financiranja: IP
ProjektŠifra: IP-2014-09-2324
Naziv (hrvatski): Patofiziološke posljedice promjena sastava lipidnih splavi
Naziv (engleski): Pathophysiological consequences of lipid raft changes
Kratica: RafTuning
Pravna nadležnost: Hrvatska
Financijer: HRZZ
Linija financiranja: IP
URL dokumentahttps://www.mdpi.com/1422-0067/22/24/13590
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje 4.0 međunarodna (CC BY 4.0)
Datum i vrijeme pohrane2022-02-01 09:24:09