prikaz prve stranice dokumenta Combined manual and automated immunophenotypisation identified disease-specific peripheral blood immune subpopulations in rheumatoid arthritis, ankylosing spondylitis and psoriatic arthritis
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Combined manual and automated immunophenotypisation identified disease-specific peripheral blood immune subpopulations in rheumatoid arthritis, ankylosing spondylitis and psoriatic arthritis
Clinical and experimental rheumatology, 38 (2020), 5; 903-916. urn:nbn:hr:105:029043
Šućur, Alan; Jajić, Zrinka; Ikić Matijašević, Marina; Stipić Marković, Asja; Flegar, Darja; Lukač, Nina; Kelava, Tomislav; Kovačić, Nataša; Grčević, Danka

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Šućur, A., Jajić, Z., Ikić Matijašević, M., Stipić Marković, A., Flegar, D., Lukač, N. ... Grčević, D. (2020). Combined manual and automated immunophenotypisation identified disease-specific peripheral blood immune subpopulations in rheumatoid arthritis, ankylosing spondylitis and psoriatic arthritis. Clinical and experimental rheumatology, 38. (5), 903-916. Preuzeto s https://urn.nsk.hr/urn:nbn:hr:105:029043

Šućur, Alan, et al. "Combined manual and automated immunophenotypisation identified disease-specific peripheral blood immune subpopulations in rheumatoid arthritis, ankylosing spondylitis and psoriatic arthritis." Clinical and experimental rheumatology, vol. 38, br. 5, 2020, str. 903-916. https://urn.nsk.hr/urn:nbn:hr:105:029043

Šućur, Alan, Zrinka Jajić, Marina Ikić Matijašević, Asja Stipić Marković, Darja Flegar, Nina Lukač, Tomislav Kelava, Nataša Kovačić i Danka Grčević. "Combined manual and automated immunophenotypisation identified disease-specific peripheral blood immune subpopulations in rheumatoid arthritis, ankylosing spondylitis and psoriatic arthritis." Clinical and experimental rheumatology 38, br. 5 (2020): 903-916. https://urn.nsk.hr/urn:nbn:hr:105:029043

Šućur, A., et al. (2020) 'Combined manual and automated immunophenotypisation identified disease-specific peripheral blood immune subpopulations in rheumatoid arthritis, ankylosing spondylitis and psoriatic arthritis', Clinical and experimental rheumatology, 38(5), str. 903-916. Preuzeto s: https://urn.nsk.hr/urn:nbn:hr:105:029043 (Datum pristupa: 11.04.2024.)

Šućur A, Jajić Z, Ikić Matijašević M, Stipić Marković A, Flegar D, Lukač N, i sur.. Combined manual and automated immunophenotypisation identified disease-specific peripheral blood immune subpopulations in rheumatoid arthritis, ankylosing spondylitis and psoriatic arthritis. Clinical and experimental rheumatology [Internet]. 10.2020. [pristupljeno 11.04.2024.];38(5):903-916. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:105:029043

A. Šućur, et al., "Combined manual and automated immunophenotypisation identified disease-specific peripheral blood immune subpopulations in rheumatoid arthritis, ankylosing spondylitis and psoriatic arthritis", Clinical and experimental rheumatology, vol. 38, br. 5, str. 903-916, Listopad 2020. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:105:029043. [Citirano: 11.04.2024.]

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Podaci o radu
Naslov (engleski)Combined manual and automated immunophenotypisation identified disease-specific peripheral blood immune subpopulations in rheumatoid arthritis, ankylosing spondylitis and psoriatic arthritis
AutorAlan Šućur
AutorZrinka Jajić
AutorMarina Ikić Matijašević
AutorAsja Stipić Marković
AutorDarja Flegar
AutorNina Lukač
AutorTomislav Kelava
AutorNataša Kovačić
AutorDanka Grčević
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
(HRVATSKI INSTITUT ZA ISTRAŽIVANJE MOZGA)
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
(KATEDRA ZA ANATOMIJU)
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
(KATEDRA ZA FIZIKALNU MEDICINU I OPĆU REHABILITACIJU)
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
(KATEDRA ZA FIZIOLOGIJU I IMUNOLOGIJU)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Temeljne medicinske znanosti
Anatomija
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Temeljne medicinske znanosti
Fiziologija čovjeka
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Kliničke medicinske znanosti
Fizikalna medicina i rehabilitacija
Sažetak (engleski)
Objectives: Rheumatoid arthritis (RA), ankylosing spondylitis (AS) and psoriatic arthritis (PsA) are associated with abnormal immune cell functions. We combined manual and automated profiling in subpopulations of T-cells, B-cells and monocytes, in parallel to functional testing and clinical correlation. ----- Methods: Using flow cytometry, we analysed the expression of CCR4, CCR6 and CXCR5 on helper and cyotoxic T-cells, CD32B and CD86 on naïve and memory B-cells, and CCR1, CCR2, CCR4 and CXCR4 on monocytes in chronic high-disease activity patients to identify peripheral blood subpopulations. Cell activation, proliferative capability and osteoclastogenic effects were tested in vitro. Comparison with synovial compartment, clinical data and anti-TNF treatment were added to peripheral blood analysis. ----- Results: PsA had lower double-negative T-cell frequency, while RA had lower double-positive T-cell frequency and expanded Th1-like and cytotoxic T-cell subsets. CD32B expression was increased on naïve and memory B-cells in AS and associated with disease activity. CCR6+ and CXCR5+ cytotoxic T-cells and CD32B+ naïve and memory B-cells were highly enriched within the synovial compartment. T-cells and B-cells from AS exhibited enhanced activation and proliferation in vitro, whereas T-cell conditioned medium from RA produced an increased osteoclastogenic effect. CCR1 and CXCR4 were upregulated on osteoclastogenic monocyte subsets of RA, AS and PsA patients. Bioinformatic Citrus analysis identified additional T-cell, B-cell and monocyte clusters specifically associated with each disease. ----- Conclusions: By combining manual and automated data analysis, our study revealed several disease-specific immune cell subpopulations, particularly cytotoxic T-cell subsets in RA and memory B-cell subsets in AS, which may serve as an indicator of active disease or possible therapeutic target.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran
Verzija publikacijeZavršna verzija rukopisa prihvaćena za objavljivanje (postprint)
Naslov časopisaClinical and experimental rheumatology
Brojčani podacivol. 38, br. 5, str. 903-916
p-ISSN0392-856X
e-ISSN1593-098X
URN:NBNurn:nbn:hr:105:029043
Datum objave publikacije2020-10
URL dokumentahttps://www.clinexprheumatol.org/abstract.asp?a=14516
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Datum isteka embarga: 2021-11-01
Uvjeti korištenjaIkona licence Zaštićeno autorskim pravom.
Datum i vrijeme pohrane2021-05-24 07:57:22